11 Biology By BISM Academy
10 – Active Site of Enzymes
Mechanism of Enzyme action.
Cataysis. Enzymes are specific. An enzyme is a three dimensional globular protein that has
specific chemical composition due to its component amino acids
a specific shape (configuration). Every enzyme by virtue of its specificity recognizes and reacts with a special chemical substance called substrate.
Any enzyme, therefore,
reacts only with its specific substrate and
transforms it into products(s).
It is then released unaltered and thus can be used again and again.
Active site Definition.
An enzyme and its substrate react with each other through a definite chargebearing site of an enzyme called active site. It may also be defined as The catalytic activity is restricted to a small portion of the structure known as the active site. Composition. The charge and shape of the active site is formed by some amino acids present in polypeptide chain of enzyme. Regions. The active site of the enzyme is made up of two definite regions i.e the binding site and the catalytic site. ENZYMES 03 Page | 6 PROF.HAMID NOOR RANA Binding site. The binding site helps the enzyme in the recognition in the recognition and binding of a proper substrate to produce an ES complex. This reaction activates the catalytic site. Catalytic site. It catalyzes the transformation of the substrate into product (s).